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BACKGROUND

Recent developments in technologies that allow the activation and inhibition of specific cell assemblies in behaving brains have revolutionized systems neuroscience.
An ideal complement to these ground-breaking tools are approaches that allow the functional imaging of large numbers of neurons in freely moving animals (e.g. head-mounted fluorescent microscopes originally developed in the Schnitzer lab). The UCLA Miniscope Project (see our wiki @
miniscope.org) has developed a series of inexpensive, open source next generation head mounted fluorescent microscopes that are being used by hundreds of labs worldwide. This workshop is sponsored by MCCS and the UCLA Integrative Center for Learning and Memory (ICLM). It is organized by the UCLA labs of Alcino Silva, Bal Khakh and Peyman Golshani.


TOPIC

This MCCS / ICLM workshop will review new ground breaking developments in an open source, new generation of head-mounted fluorescent microscopes (miniscopes). For a wiki describing these miniscopes, associated protocols, part numbers etc, go to miniscope.org. The long-term goal is to continue to foster a wiki-like community of users that will share the development of this powerful technology, from the design of the miniscopes to the computer software, surgery methods, etc, in an open source and commercial free environment. This workshop is part of a series of events to share the evolving technology of miniscopes. The workshop will be on Thursday November 1, 2018 (1-5:30 PM; right before the SFN meeting) in the UCSD Medical Education and Telemedicine Building - Lower Auditorium


WORKSHOP SCHEDULE

November 1, 2018 (1-5 :30 PM)
University of California, San Diego (UCSD)
Medical Education and Telemedicine Building - Lower Auditorium

1:05-1:15 Welcome: Alcino Silva (UCLA) and Peyman Golshami (UCLA)

1:15-1:25
Overview: Denise Cai (Mt Sinai)

1:25-1:55
Imaging principles: Daniel Aharoni (UCLA)

1:55-2:25
Implantation & baseplate surgeries: Tristan Shuman (Mt Sinai)

2:25-3:05
Assembly & imaging: Denise Cai (Mt Sinai)

3:05-3:35
Imaging analyses: Pengcheng Zhou (Columbia U)

3:35-3:40
Imaging MSNs and data analyses: Xinzhu Yu (UCLA)

3:40-3:45
Imaging neocortex: Megha Sehgal (UCLA)

3:45- 3:50
Imaging deep structures: Pingping Zhao (UCLA)

3:50-4:00
BREAK

4:00 -4:15
Imaging rat CA1: Tad Blair (UCLA)

4:15-4:30
Integrating silicon probes with miniscopes: Sotiris Masmanidis (UCLA)

4:30- 4:45
Light field miniscope: Alipasha Vaziri (Rockefeller U)

4:45- 5:00
Future developments in Miniscopes: Daniel Aharoni (UCLA)

5:00-5:30
General Q&A and Miniscope Raffle


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Because of the limited availability of openings in this workshop, registration is mandatory.